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A seed-stock system ought to be employed for very long-term storage of cultures. Cultures received in the ATCC must be resuscitated according to Instructions. If grown in broth, the cells are pelleted by centrifugation. Resuspend in one/twentieth the volume of contemporary maintenance broth, and increase an equivalent volume of twenty% (v/v in drin

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Whilst the main aim of freeze-drying is moisture elimination, guaranteeing that the minute amounts left driving—residual moisture—are in suitable restrictions is crucial. Why? This seemingly insignificant moisture can have a big influence on solution balance and longevity.While challenges including solution sensitivity and contamination challen